Lesson 4 Extension Activity: Calculate Transformation Efficiency
HYPOTHESIS:
SOLVED: Calculate the DNA concentration and transformation efficiency for below scenario: 50 ng of DNA is ligated in a 20 μL reaction. After ligation, the reaction is diluted 2-fold and 5 μL
SOLVED: Exercise C: Calculating transformation efficiency Table 1 . Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA DNA Diluted? added? Y or N Volume Concentration Total DNA
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